UJI AKTIVITAS ANTIBAKTERI EKSTRAK DAUN SIRIH HIJAU (Piper betle L.) TERHADAP BAKTERI Pseudomonas aeruginosa
Abstract
The escalating crisis of antibiotic resistance necessitates the exploration of novel antibacterial agents from natural sources. Green betel leaf (Piper betle L.) is a traditional medicinal plant empirically known for its antiseptic properties. This study aimed to scientifically evaluate the antibacterial activity of its ethanol extract against the opportunistic pathogen Pseudomonas aeruginosa. Extraction was performed via maceration with 96% ethanol, followed by phytochemical screening and antibacterial testing using the disc diffusion method at concentrations of 15%, 20%, and 25% (w/v). Chloramphenicol and 96% ethanol served as positive and negative controls, respectively. Phytochemical analysis confirmed the presence of phenolics, tannins, alkaloids, and terpenoids. The extract exhibited antibacterial activity against P. aeruginosa, with inhibition zones of 3.32 mm, 2.58 mm, and 3.59 mm for the 15%, 20%, and 25% concentrations, respectively, categorizing all as "moderate" activity. While significantly lower than the positive control (22.37 mm), this activity confirms the extract's potential. The highest activity at the 25% concentration suggests a dose-dependent relationship. These findings support the traditional use of betel leaf and provide a scientific basis for its development as a complementary natural antibacterial agent against resilient pathogens like P. aeruginosa.
References
Dewi, G. P., Kuntorini, E. M., & Pujawati, E. D. (2020). Struktur Anatomi dan Uji Histokimia Terpenoid dan Fenol Dua Varietas Sirih Hijau (Piper betle L.). Bioscientiae, 17(2), 1–14.
Hutauruk, D., Marpaung, J. K., Rumela, N., Laia, & Ginting, J. A. (2025). Effectiveness Test of Green Betel Leaf Ethanol Extract Gel Preparation (Piper betle L.) Against Pseudomonas aeruginosa Bacteria. Journal of Innovative and Creativity, 5(2), 13318–13323.
Magvirah, T., Marwati, & Ardhani, F. (2019). Uji Daya Hambat Bakteri Staphylococcus aureus Menggunakan Ekstrak Daun Tahongai (Kleinhovia hospita L.). Jurnal Peternakan Lingkungan Tropis, 2(2), 41–50.
Manarisip, G. E., Fatimawali, & Rotinsulu, H. (2020). Standarisasi Ekstrak Daun Sirih Hijau (Piper betle L.) dan Uji Antibakteri Terhadap Bakteri Pseudomonas aeruginosa. Pharmacon, 9(4), 533–541.
Mukafiah, N., & Sulistyani, N. (2022). Study of Antibacterial Activity of Green Betel Leaf (Piper betle L.) and Red Betel Leaf (Piper crocatum L.) Extract Against Pseudomonas aeruginosa Bacteria. Farmasains: Jurnal Farmasi dan Ilmu Kesehatan, 7(1), 47–56.
Nayaka, N. M. D. M. W., et al. (2021). Piper betle (L): Recent Review of Antibacterial and Antifungal Properties, Safety Profiles, and Commercial Applications. Molecules, 26(2321), 1–21.
Nisyak, K., Hisbiyah, A., & Haqqo, A. (2022). Aktivitas Antibakteri Ekstrak Etanol dan Minyak Atsiri Sirih Hijau Terhadap Methicillin Resistant Staphylococcus aureus. J-PhAM Journal of Pharmaceutical Care Anwar Medika, 5(1), 1–14.
Oliveira, C., Silva, L., & Sousa, D. (2020). Mechanisms of Pseudomonas aeruginosa Antibiotic Resistance. Antibiotics, 9(7), 452.
Sadiah, H. H., Cahyadi, A. I., & Windria, S. (2022). Kajian Potensi Daun Sirih Hijau (Piper betle L) sebagai Antibakteri. Jurnal Sain Veteriner, 40(2), 128–138.
Septiani, L. E., Atmodjo, P. K., & Sidharta, B. B. R. (2024). Kandungan Metabolit dan Aktivitas Antibakteri Minyak Atsiri Daun Sirih Hijau (Piper betle L.) dan Sirih Merah (Piper crocatum) Terhadap Pseudomonas aeruginosa dan Staphylococcus aureus. Prosiding SENAPAS, 2(1), 87–92.
